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MMP19 Polyclonal antibody

MMP19 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14244-1-AP

Print datasheet

Synonyms

Matrix metalloproteinase-19, Matrix metalloproteinase-18, Matrix metalloproteinase 19, Matrix metalloproteinase 18, matrix metallopeptidase 19

验证数据展示

  • WB analysis of SH-SY5Y using 14244-1-AP

    WB analysis of SH-SY5Y using 14244-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14244-1-AP (MMP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14244-1-AP

    IHC staining of human liver cancer using 14244-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14244-1-AP (MMP19 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of SH-SY5Y using 14244-1-AP

    IF Staining of SH-SY5Y using 14244-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using MMP19 antibody (14244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of human placenta using 14244-1-AP

    IP experiment of human placenta using 14244-1-AP

    IP result of anti-MMP19 (IP:14244-1-AP, 4ug; Detection:14244-1-AP 1:500) with human placenta tissue lysate 1520ug.

经过测试的应用

Positive WB detected inSH-SY5Y cells, human placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14244-1-AP targets MMP19 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen MMP19 fusion protein Ag5501 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 19
Synonyms Matrix metalloproteinase-19, Matrix metalloproteinase-18, Matrix metalloproteinase 19, Matrix metalloproteinase 18, matrix metallopeptidase 19
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57-60 kDa
GenBank Accession NumberBC050368
Gene Symbol MMP19
Gene ID (NCBI) 4327
RRIDAB_10637857
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99542
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP19(matrix metalloproteinase-19) has the capacity to degrade several basement membrane proteins such as type IV collagen, laminin, tenascin C, and nidogen. It is expressed by microvascular endothelial cells in vitro as well as by capillaries in the inflamed synovial membrane, whereas its expression is absent in quiescent macrovascular endothelial cells of large arteries and veins . And it is also detected in capillaries of normal mammary tissue, benign mammary carcinomas and in skin capillaries(PMID:15241558).

实验方案

Product Specific Protocols
WB protocol for MMP19 antibody 14244-1-APDownload protocol
IHC protocol for MMP19 antibody 14244-1-APDownload protocol
IF protocol for MMP19 antibody 14244-1-APDownload protocol
IP protocol for MMP19 antibody 14244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Bioact Mater

Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis

Authors - Wei Zhang
View Article
mouseWB

Cancer Gene Ther

A critical ETV4/Twist1/Vimentin axis in Ha-RAS-induced aggressive breast cancer.

Authors - Wuling Liu
View Article
humanWB,IHC

J Cell Biochem

MicroRNA-193b-3p Regulates Matrix Metalloproteinase 19 Expression in Interleukin-1β-Induced Human Chondrocytes.

Authors - Zong-Kun Chang
View Article
humanWB

Front Pharmacol

METTL3/m6A/IFIT2 regulates proliferation, invasion and immunity in esophageal squamous cell carcinoma

Authors - Fangfang Ge
View Article
humanWB

J Biochem Mol Toxicol

Pacenta polypeptide injection alleviates the fibrosis and inflammation in cigarette smoke extracts-induced BEAS-2B cells by modulating MMP-9/TIMP-1 signaling

Authors - Wei Xu
View Article
ratWB

J Immunol Res

Dissecting the Molecular Mechanism of Wang-Bi Capsule in the Treatment of Experimental Rheumatoid Arthritis Based on Synovial Tissue Proteomic Analysis.

Authors - Haiyang Shu
View Article