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CoraLite® Plus 488-conjugated MMP19 Polyclonal antibody

MMP19 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14244

Print datasheet

Synonyms

matrix metallopeptidase 19, Matrix metalloproteinase 18, Matrix metalloproteinase 19, Matrix metalloproteinase RASI, MMP 18, MMP 19, MMP18, MMP19, RASI, RASI 1

验证数据展示

  • IF Staining of SH-SY5Y using CL488-14244

    IF Staining of SH-SY5Y using CL488-14244

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 MMP19 antibody (CL488-14244) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 14244-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14244 targets MMP19 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen MMP19 fusion protein Ag5501 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 19
Synonyms matrix metallopeptidase 19, Matrix metalloproteinase 18, Matrix metalloproteinase 19, Matrix metalloproteinase RASI, MMP 18, MMP 19, MMP18, MMP19, RASI, RASI 1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57-60 kDa
GenBank Accession NumberBC050368
Gene Symbol MMP19
Gene ID (NCBI) 4327
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99542
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP19(matrix metalloproteinase-19) has the capacity to degrade several basement membrane proteins such as type IV collagen, laminin, tenascin C, and nidogen. It is expressed by microvascular endothelial cells in vitro as well as by capillaries in the inflamed synovial membrane, whereas its expression is absent in quiescent macrovascular endothelial cells of large arteries and veins . And it is also detected in capillaries of normal mammary tissue, benign mammary carcinomas and in skin capillaries(PMID:15241558).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MMP19 antibody CL488-14244Download protocol
Standard Protocols
Click here to view our Standard Protocols