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MMP3 Recombinant antibody, PBS Only (Detector)

MMP3 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

230535G8

Cat no : 82984-4-PBS

Print datasheet

Synonyms

SL-1, MMP-3, MMP 3, Matrix metalloproteinase-3, EC:3.4.24.17

验证数据展示

  • WB analysis using 82984-4-RR (same clone as 82984-4-PBS)

    WB analysis using 82984-4-RR (same clone as 82984-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82984-4-RR (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82984-4-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82984-4-RR (same clone as 82984-4-PBS)

    FC experiment of HepG2 using 82984-4-RR (same clone as 82984-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MMP3 Recombinant antibody (82984-4-RR, Clone:230535G8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82984-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00083-3

    Cytometric bead array standard curve of MP00083-3

    Cytometric bead array standard curve ofMP00083-3, MMP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82984-1-PBS. Detection antibody: 82984-4-PBS. Standard: Eg0382. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 82984-4-RR

    Affinity and Kinetic Characterization of 82984-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82984-4-RR against Human MMP3 were performed. The affinity constant is 0.348 nM.

Planning an IHC experiment? We recommend our IHCeasy MMP3 Ready-To-Use IHC Kit. MMP3 primary antibody included.

产品信息

82984-4-PBS targets MMP3 as part of a matched antibody pair:

MP00083-3: 82984-1-PBS capture and 82984-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Indirect ELISA, WB Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Synonyms SL-1, MMP-3, MMP 3, Matrix metalloproteinase-3, EC:3.4.24.17
GenBank Accession NumberBC074815
Gene Symbol MMP3
Gene ID (NCBI) 4314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08254
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C