验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy MMP3 Ready-To-Use IHC Kit. MMP3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66338-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66338 targets MMP3 in IF/ICC applications and shows reactivity with human, rat, mouse, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, rat, mouse, pig |
| 免疫原 | MMP3 fusion protein Ag12359 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | matrix metallopeptidase 3 (stromelysin 1, progelatinase) |
| 别名 | CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1 |
| 计算分子量 | 477 aa, 54 kDa |
| 观测分子量 | 45-60 kDa |
| GenBank蛋白编号 | BC074869 |
| 基因名称 | MMP3 |
| Gene ID (NCBI) | 4314 |
| RRID | AB_2919311 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P08254 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MMP3 antibody CL488-66338 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |