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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated MOV10 Polyclonal antibody

MOV10 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10370

Print datasheet

Synonyms

KIAA1631, Helicase MOV-10, gb110, fSAP113, EC:3.6.4.13

验证数据展示

  • IF Staining of HepG2 using CL594-10370

    IF Staining of HepG2 using CL594-10370

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 MOV10 antibody (CL594-10370) at dilution of 1:200.

  • FC experiment of HeLa using CL594-10370

    FC experiment of HeLa using CL594-10370

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated MOV10 Polyclonal antibody (CL594-10370)(red), or 0.4 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MOV10 Ready-To-Use IHC Kit. MOV10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10370-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10370 targets MOV10 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen MOV10 fusion protein Ag0384 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Mov10, Moloney leukemia virus 10, homolog (mouse)
Synonyms KIAA1631, Helicase MOV-10, gb110, fSAP113, EC:3.6.4.13
Calculated Molecular Weight 114 kDa
Observed Molecular Weight 110-115 kDa
GenBank Accession NumberBC002548
Gene Symbol MOV10
Gene ID (NCBI) 4343
RRIDAB_2919731
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCE1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.

实验方案

Product Specific Protocols
IF protocol for CL594 MOV10 antibody CL594-10370Download protocol
FC protocol for CL594 MOV10 antibody CL594-10370Download protocol
Standard Protocols
Click here to view our Standard Protocols