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MPHOSPH6 Polyclonal antibody

MPHOSPH6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10695-1-AP

Print datasheet

Synonyms

M phase phosphoprotein 6, MPHOSPH6, MPP, MPP 6, MPP6

验证数据展示

  • WB analysis of MCF-7 using 10695-1-AP

    WB analysis of MCF-7 using 10695-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10695-1-AP

    IHC staining of human breast cancer using 10695-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of HeLa using 10695-1-AP

    IP experiment of HeLa using 10695-1-AP

    IP result of anti-MPHOSPH6 (IP:10695-1-AP, 4ug; Detection:10695-1-AP 1:500) with HeLa cells lysate 3000 ug.

经过测试的应用

Positive WB detected inMCF7 cells, HeLa cells, MCF-7 cells, MDA-MB-453s cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10695-1-AP targets MPHOSPH6 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MPHOSPH6 fusion protein Ag1080 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name M-phase phosphoprotein 6
Synonyms M phase phosphoprotein 6, MPHOSPH6, MPP, MPP 6, MPP6
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC005242
Gene Symbol MPHOSPH6
Gene ID (NCBI) 10200
RRIDAB_2282161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99547
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MPHOSPH6 antibody 10695-1-APDownload protocol
IHC protocol for MPHOSPH6 antibody 10695-1-APDownload protocol
IP protocol for MPHOSPH6 antibody 10695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell Prolif

The 5.8S pre-rRNA maturation factor, M-phase phosphoprotein 6, is a female fertility factor required for oocyte quality and meiosis.

Authors - Rui-Rui Peng
View Article