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  • Featured Product
  • KD/KO Validated

MPO Monoclonal antibody, PBS Only

MPO Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgA

Reactivity

human, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C11F6

Cat No : 66177-1-PBS

Print datasheet

Synonyms

MPO, myeloperoxidase

验证数据展示

  • IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MPO antibody included
  • IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MPO antibody included
  • IF Staining of human tonsillitis using 66177-1-Ig (same clone as 66177-1-PBS)

    IF Staining of human tonsillitis using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66177-1-Ig (MPO antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • WB analysis of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    WB analysis of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66177-1-Ig (MPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

Not recommend for IHC about rat sample. Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy MPO Ready-To-Use IHC Kit. MPO primary antibody included.

产品信息

66177-1-PBS targets MPO in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen MPO fusion protein Ag17564 种属同源性预测
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Full Name myeloperoxidase
Synonyms MPO, myeloperoxidase
Calculated Molecular Weight 745 aa, 84 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC130476
Gene Symbol MPO
Gene ID (NCBI) 4353
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP05164
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.