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验证数据展示

  • IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MPO antibody included
  • IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MPO antibody included
  • WB analysis of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    WB analysis of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66177-1-Ig (MPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • FC experiment of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    FC experiment of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    1x10^6 HL-60 cells were intracellularly stained with 0.25 ug MPO Monoclonal antibody (66177-1-Ig, Clone:4C11F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • IF Staining of human appendicitis using 66177-1-Ig (same clone as 66177-1-PBS)

    IF Staining of human appendicitis using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using MPO antibody (66177-1-Ig, Clone: 4C11F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • IF Staining of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    IF Staining of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (66177-1-Ig, Clone: 4C11F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MPO Monoclonal antibody, PBS Only

MPO Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 66177-1-PBS

产品说明书

CloneNo. 4C11F6

宿主/亚型

Mouse / IgA

种属反应性

human, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

4C11F6, EC:1.11.2.2, myeloperoxidase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Not recommend for IHC about rat sample. Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy MPO Ready-To-Use IHC Kit. MPO primary antibody included.

产品信息

66177-1-PBS targets MPO in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 MPO fusion protein Ag17564 种属同源性预测
宿主/亚型 Mouse / IgA
抗体类别 Monoclonal
产品类型 Antibody
全称 myeloperoxidase
别名 4C11F6, EC:1.11.2.2, myeloperoxidase
计算分子量 745 aa, 84 kDa
观测分子量 90 kDa
GenBank蛋白编号BC130476
基因名称 MPO
Gene ID (NCBI) 4353
RRIDAB_2881572
偶联类型 Unconjugated
形式 Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDP05164
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.