- Featured Product
- KD/KO Validated
MPO Monoclonal antibody
MPO Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgA
Reactivity
human, rat and More (2)
Applications
WB, IHC, IF/ICC, IF-P, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
4C11F6
验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells |
Positive IHC detected in | human liver tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human appendicitis tissue |
Positive IF/ICC detected in | HL-60 cells |
Not recommend for IHC about rat sample. Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66177-1-Ig targets MPO in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, ELISA |
Tested Reactivity | human, rat |
Cited Reactivity | human, rat, bovine, cow |
Immunogen | MPO fusion protein Ag17564 种属同源性预测 |
Host / Isotype | Mouse / IgA |
Class | Monoclonal |
Type | Antibody |
Full Name | myeloperoxidase |
Synonyms | myeloperoxidase, EC:1.11.2.2, 4C11F6 |
Calculated Molecular Weight | 745 aa, 84 kDa |
Observed Molecular Weight | 90 kDa |
GenBank Accession Number | BC130476 |
Gene Symbol | MPO |
Gene ID (NCBI) | 4353 |
RRID | AB_2881572 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Caprylic acid/ammonium sulfate precipitation |
UNIPROT ID | P05164 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for MPO antibody 66177-1-Ig | Download protocol |
IHC protocol for MPO antibody 66177-1-Ig | Download protocol |
IF protocol for MPO antibody 66177-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
PLoS Pathog Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice. | ||
Int Immunopharmacol Sappanone A enhances hepatocyte proliferation in lipopolysaccharide-induced acute liver injury in mice by promoting injured hepatocyte apoptosis and regulating macrophage polarization | ||
FEBS J A combined model of serum neutrophil extracellular traps, CD8+ T cells, and tumor proportion score provides better prediction of PD-1 inhibitor efficacy in patients with NSCLC | ||
Sci Rep Persisting and Increasing Neutrophil Infiltration Associates with Gastric Carcinogenesis and E-cadherin Downregulation. | ||
Sci Rep Interleukin 22 attenuated angiotensin II induced acute lung injury through inhibiting the apoptosis of pulmonary microvascular endothelial cells. | ||
Front Immunol Leukocytes in Cerebral Thrombus Respond to Large-Vessel Occlusion in a Time-Dependent Manner and the Association of NETs With Collateral Flow. |