验证数据展示
经过测试的应用
| Positive IF-P detected in | human appendicitis tissue, human liver tissue |
| Positive FC (Intra) detected in | HL-60 cells |
Not recommend for IHC about rat sample. Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-66177 targets MPO in IF-P, FC (Intra) applications and shows reactivity with human, rat samples.
| 经测试应用 | IF-P, FC (Intra) Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 | MPO fusion protein Ag17564 种属同源性预测 |
| 宿主/亚型 | Mouse / IgA |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | myeloperoxidase |
| 别名 | myeloperoxidase, EC:1.11.2.2, 4C11F6 |
| 计算分子量 | 745 aa, 84 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC130476 |
| 基因名称 | MPO |
| Gene ID (NCBI) | 4353 |
| RRID | AB_2919960 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Thiophilic affinity chromatograph |
| UNIPROT ID | P05164 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 MPO antibody CL594-66177 | Download protocol |
| FC protocol for CL594 MPO antibody CL594-66177 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
