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CoraLite®594-conjugated MPO Monoclonal antibody
MPO Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgA
Reactivity
human, rat
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
4C11F6
验证数据展示
经过测试的应用
Positive IF detected in | human tonsillitis tissue, human liver tissue |
Not recommend for IHC about rat sample. Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Planning an IHC experiment? We recommend our IHCeasy MPO Ready-To-Use IHC Kit. MPO primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66177-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66177 targets MPO in IF applications and shows reactivity with human, rat samples.
Tested Applications | IF-P Application Description |
Tested Reactivity | human, rat |
Immunogen | MPO fusion protein Ag17564 种属同源性预测 |
Host / Isotype | Mouse / IgA |
Class | Monoclonal |
Type | Antibody |
Full Name | myeloperoxidase |
Synonyms | MPO, myeloperoxidase |
Calculated Molecular Weight | 745 aa, 84 kDa |
Observed Molecular Weight | 90 kDa |
GenBank Accession Number | BC130476 |
Gene Symbol | MPO |
Gene ID (NCBI) | 4353 |
RRID | AB_2919960 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Thiophilic affinity chromatograph |
UNIPROT ID | P05164 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 MPO antibody CL594-66177 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |