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MPO Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 39-2500 pg/mL

Cat No : MP00321-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00321-2, Human MPO Recombinant Matched Antibody Pair - PBS only. 83282-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0511. 83282-5-PBS was HRP conjugated as the detection antibody. Range: 39-2500 pg/mL.

    Sandwich ELISA standard curve of MP00321-2

    Sandwich ELISA standard curve of MP00321-2, Human MPO Recombinant Matched Antibody Pair - PBS only. 83282-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0511. 83282-5-PBS was HRP conjugated as the detection antibody. Range: 39-2500 pg/mL.

  • Serum of sixteen individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 169.9 ng/mL with a range of 54.5 - 419.2 ng/mL.

    Sample test of MP00321-2

    Serum of sixteen individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 169.9 ng/mL with a range of 54.5 - 419.2 ng/mL.

  • Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean MPO concentration was determined to be 6.6 ng/mL in unstimulated PBMC supernatant, 20.0 ng/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean MPO concentration was determined to be 7.2 ng/mL in unstimulated PBMC supernatant, 28.2 ng/mL in PHA stimulated PBMC supernatant.

    Sample test of MP00321-2

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean MPO concentration was determined to be 6.6 ng/mL in unstimulated PBMC supernatant, 20.0 ng/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean MPO concentration was determined to be 7.2 ng/mL in unstimulated PBMC supernatant, 28.2 ng/mL in PHA stimulated PBMC supernatant.

  • Urine of six individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 2,249.9 pg/mL with a range of 294.1 - 6,991.1 pg/mL.

    Sample test of MP00321-2

    Urine of six individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 2,249.9 pg/mL with a range of 294.1 - 6,991.1 pg/mL.

  • Saliva of eight individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 314.7 ng/mL with a range of 6.3 - 1,446.1 ng/mL.

    Sample test of MP00321-2

    Saliva of eight individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 314.7 ng/mL with a range of 6.3 - 1,446.1 ng/mL.

  • The mean MPO concentration was determined to be 73,583.3 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

    Sample test of MP00321-2

    The mean MPO concentration was determined to be 73,583.3 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00321-2 targets MPO in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 39-2500 pg/mL (Sandwich ELISA);

Capture antibody: MPO Recombinant antibody, PBS Only (Capture) 83282-4-PBS (240229G6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: MPO Recombinant antibody, PBS Only (Detector) 83282-5-PBS (240229A6). 100 μg. Concentration 1 mgl/ml.

Alternative MPO matched antibody pairs: MP00321-1, MP50164-1

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83282-4-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myeloperoxidase
Synonyms myeloperoxidase, EC:1.11.2.2, 240229G6
GenBank accession numberBC130476
Gene symbol MPO
UniprotP05164
Gene ID (NCBI) 4353
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83282-5-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myeloperoxidase
Synonyms myeloperoxidase, EC:1.11.2.2, 240229A6
GenBank accession numberBC130476
Gene symbol MPO
UniprotP05164
Gene ID (NCBI) 4353
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C