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MPP2 Polyclonal antibody

MPP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13589-1-AP

Print datasheet

Synonyms

Discs large homolog 2, DLG2, MAGUK p55 subfamily member 2, MPP2, Protein MPP2

验证数据展示

  • WB analysis of mouse eye using 13589-1-AP

    WB analysis of mouse eye using 13589-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13589-1-AP (MPP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 13589-1-AP

    WB analysis using 13589-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13589-1-AP (MPP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse eye using 13589-1-AP

    IP experiment of mouse eye using 13589-1-AP

    IP result of anti-MPP2 (IP:13589-1-AP, 4ug; Detection:13589-1-AP 1:2000) with mouse eye tissue lysate 2260 ug.

  • IHC staining of mouse brain using 13589-1-AP

    IHC staining of mouse brain using 13589-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13589-1-AP (MPP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13589-1-AP

    IHC staining of mouse brain using 13589-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13589-1-AP (MPP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse eye tissue, mouse retina tissue
Positive IP detected inmouse eye tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13589-1-AP targets MPP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MPP2 fusion protein Ag4223 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
Synonyms Discs large homolog 2, DLG2, MAGUK p55 subfamily member 2, MPP2, Protein MPP2
Calculated Molecular Weight 576 aa, 65 kDa
Observed Molecular Weight 65 kDa, 60 kDa
GenBank Accession NumberBC030287
Gene Symbol MPP2
Gene ID (NCBI) 4355
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14168
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Membrane protein palmitoylated 2 (MPP2) is a member of the membrane-associated guanylate kinase (MAGUK) family. MPPs can form diverse protein complexes at the cell membranes and play an important role in cell structure, polarity and cell adhesion. MPP2 is a novel postsynaptic scaffold protein that binds to the synaptic cell adhesion molecule SynCAM1. MPP2 also is a component of AMPA receptor-associated protein complexes.

实验方案

Product Specific Protocols
WB protocol for MPP2 antibody 13589-1-APDownload protocol
IHC protocol for MPP2 antibody 13589-1-APDownload protocol
IP protocol for MPP2 antibody 13589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols