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MPP8 Recombinant antibody, PBS Only

MPP8 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242205B5

Cat no : 84719-4-PBS

Print datasheet

Synonyms

MPHOSPH8, Two hybrid-associated protein 3 with RanBPM, Twa3, M-phase phosphoprotein 8, HSMPP8

验证数据展示

  • WB analysis using 84719-4-RR (same clone as 84719-4-PBS)

    WB analysis using 84719-4-RR (same clone as 84719-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84719-4-RR (MPP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84719-4-PBS in a different storage buffer formulation.

产品信息

84719-4-PBS targets MPP8 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MPP8 fusion protein Ag10268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name M-phase phosphoprotein 8
Synonyms MPHOSPH8, Two hybrid-associated protein 3 with RanBPM, Twa3, M-phase phosphoprotein 8, HSMPP8
Calculated Molecular Weight 860 aa, 97 kDa
Observed Molecular Weight 97-105 kDa
GenBank Accession NumberBC003542
Gene Symbol MPP8
Gene ID (NCBI) 54737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ99549
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The M-phase phosphoprotein 8 (MPP8) was firstly identified to coimmunoprecipitate with the RanBPM-comprised large protein complex [PMID:19956676]. It also binds to methylated H3K9 and recruited the H3K9 methyltransferases GLP and ESET, as well as DNA methyltransferase 3A (DNMT3A) to the promoter of the E-cadherin gene, a key regulator of tumor cell growth and epithelial-to-mesenchymal transition (EMT) [PMID:18726070]. This binding event of MPP8-H3K9me modulates the expression of E-cadherin, a key regulator of cell-cell adhesion with implications in embryonic development, tissue morphogenesis and homeostasis , together with H3K9 methyltransferases GLP and ESET and DNA methyltransferase 3a (Dnmt3a)