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验证数据展示

  • WB analysis using 84719-4-RR

    WB analysis using 84719-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84719-4-RR (MPP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

MPP8 Recombinant antibody

MPP8 Recombinant Antibody for WB, ELISA
Cat No. 84719-4-RR

Print datasheet

CloneNo. 242205B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MPHOSPH8, Two hybrid-associated protein 3 with RanBPM, Twa3, M-phase phosphoprotein 8, HSMPP8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84719-4-RR targets MPP8 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen MPP8 fusion protein Ag10268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name M-phase phosphoprotein 8
Synonyms MPHOSPH8, Two hybrid-associated protein 3 with RanBPM, Twa3, M-phase phosphoprotein 8, HSMPP8
Calculated Molecular Weight 860 aa, 97 kDa
Observed Molecular Weight 97-105 kDa
GenBank Accession NumberBC003542
Gene Symbol MPP8
Gene ID (NCBI) 54737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ99549
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The M-phase phosphoprotein 8 (MPP8) was firstly identified to coimmunoprecipitate with the RanBPM-comprised large protein complex [PMID:19956676]. It also binds to methylated H3K9 and recruited the H3K9 methyltransferases GLP and ESET, as well as DNA methyltransferase 3A (DNMT3A) to the promoter of the E-cadherin gene, a key regulator of tumor cell growth and epithelial-to-mesenchymal transition (EMT) [PMID:18726070]. This binding event of MPP8-H3K9me modulates the expression of E-cadherin, a key regulator of cell-cell adhesion with implications in embryonic development, tissue morphogenesis and homeostasis , together with H3K9 methyltransferases GLP and ESET and DNA methyltransferase 3a (Dnmt3a)

实验方案

Product Specific Protocols
WB protocol for MPP8 antibody 84719-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols