MRE11A Monoclonal antibody

MRE11A Monoclonal Antibody for WB, ELISA
Cat No. 67570-1-Ig

产品说明书

CloneNo. 1B12B2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, ELISA

ATLD, HNGS1, MRE11, MRE11 homolog 1, MRE11 homolog A, MRE11A, MRE11B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:3000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67570-1-Ig targets MRE11A in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 MRE11A fusion protein Ag28847 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
别名 ATLD, HNGS1, MRE11, MRE11 homolog 1, MRE11 homolog A, MRE11A, MRE11B
观测分子量 81 kDa
GenBank蛋白编号BC063458
基因名称 MRE11A
Gene ID (NCBI) 4361
RRIDAB_2882784
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49959
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mre11 (meiotic recombination 11), also named as Mre11A, is a component of the MRN( MRE11/RAD50/NBS1) complex which is a versatile complex, playing a key role in the sensing, processing, and repair of DSBs. MRE11 possesses both endonuclease and 3'-5' exonuclease activity. It contains two DNA-binding domains (DBDs), enabling it to bind both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with CtIP (CtBP [C terminal binding protein] interacting protein) to initiate end resection. It's reported that MRE11 is over-expressed in breast cancers. (PMID:38128537; 22914783)

实验方案

Product Specific Protocols
WB protocol for MRE11A antibody 67570-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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