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验证数据展示

  • WB analysis using 68467-1-Ig

    WB analysis using 68467-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68467-1-Ig (MRPL28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

MRPL28 Monoclonal antibody

MRPL28 Monoclonal Antibody for WB, ELISA
Cat No. 68467-1-Ig

产品说明书

CloneNo. 2E6B2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

L28mt, MAAT1, Melanoma antigen p15, MRP L28, MRPL28, p15

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH-3T3 cells, 4T1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68467-1-Ig targets MRPL28 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 MRPL28 fusion protein Ag33198 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L28
别名 L28mt, MAAT1, Melanoma antigen p15, MRP L28, MRPL28, p15
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC000990
基因名称 MRPL28
Gene ID (NCBI) 10573
RRIDAB_3085178
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13084
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL28 antibody 68467-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols