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MRPL35 Polyclonal antibody

MRPL35 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27339-1-AP

Print datasheet

Synonyms

MRPL 35, MRP L35, L35mt

验证数据展示

  • WB analysis using 27339-1-AP

    WB analysis using 27339-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27339-1-AP (MRPL35 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 27339-1-AP

    IF Staining of HepG2 using 27339-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL35 antibody (27339-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27339-1-AP targets MRPL35 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MRPL35 fusion protein Ag26300 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L35
Synonyms MRPL 35, MRP L35, L35mt
Calculated Molecular Weight 170 aa, 19 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC020651
Gene Symbol MRPL35
Gene ID (NCBI) 51318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZE8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial ribosomal protein L35 (MRPL35) is a member of the large subunit family of mitochondrial ribosomal proteins. It plays a key role in the assembly of cytochrome C oxidase, involved in the translation of mitochondrial proteins.

实验方案

Product Specific Protocols
WB protocol for MRPL35 antibody 27339-1-APDownload protocol
IF protocol for MRPL35 antibody 27339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols