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验证数据展示

  • WB analysis using 16767-1-AP

    WB analysis using 16767-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16767-1-AP (MRPL40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16767-1-AP

    IP experiment of HeLa using 16767-1-AP

    IP result of anti-MRPL40 (IP:16767-1-AP, 4ug; Detection:16767-1-AP 1:2000) with HeLa cells lysate 1920 ug.

  • IHC staining of human ovary cancer using 16767-1-AP

    IHC staining of human ovary cancer using 16767-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16767-1-AP (MRPL40 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 16767-1-AP

    IF Staining of HeLa using 16767-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL40 antibody (16767-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

MRPL40 Polyclonal antibody

MRPL40 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16767-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

NLVCF, MRP-L40, MRP L40, MRP L22, Large ribosomal subunit protein mL40

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16767-1-AP targets MRPL40 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 MRPL40 fusion protein Ag10341 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L40
别名 NLVCF, MRP-L40, MRP L40, MRP L22, Large ribosomal subunit protein mL40
计算分子量 206 aa, 24 kDa
观测分子量18-24 kDa
GenBank蛋白编号BC009707
基因名称 MRPL40
Gene ID (NCBI) 64976
RRIDAB_3669256
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ50
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL40 antibody 16767-1-APDownload protocol
IHC protocol for MRPL40 antibody 16767-1-APDownload protocol
IF protocol for MRPL40 antibody 16767-1-APDownload protocol
IP protocol for MRPL40 antibody 16767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols