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MRPL40 Polyclonal antibody

MRPL40 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16767-1-AP

Print datasheet

Synonyms

NLVCF, MRP-L40, MRP L40, MRP L22, Large ribosomal subunit protein mL40

验证数据展示

  • WB analysis using 16767-1-AP

    WB analysis using 16767-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16767-1-AP (MRPL40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16767-1-AP

    IP experiment of HeLa using 16767-1-AP

    IP result of anti-MRPL40 (IP:16767-1-AP, 4ug; Detection:16767-1-AP 1:2000) with HeLa cells lysate 1920 ug.

  • IHC staining of human ovary cancer using 16767-1-AP

    IHC staining of human ovary cancer using 16767-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16767-1-AP (MRPL40 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16767-1-AP targets MRPL40 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human
Immunogen MRPL40 fusion protein Ag10341 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L40
Synonyms NLVCF, MRP-L40, MRP L40, MRP L22, Large ribosomal subunit protein mL40
Calculated Molecular Weight 206 aa, 24 kDa
Observed Molecular Weight18-24 kDa
GenBank Accession NumberBC009707
Gene Symbol MRPL40
Gene ID (NCBI) 64976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ50
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL40 antibody 16767-1-APDownload protocol
IHC protocol for MRPL40 antibody 16767-1-APDownload protocol
IP protocol for MRPL40 antibody 16767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols