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验证数据展示

  • WB analysis using 17213-1-AP

    WB analysis using 17213-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17213-1-AP (MRPL50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17213-1-AP

    IHC staining of human liver using 17213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17213-1-AP (MRPL50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRPL50 Polyclonal antibody

MRPL50 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17213-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

L50mt, MRP L50, MRPL50

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17213-1-AP targets MRPL50 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 MRPL50 fusion protein Ag10996 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L50
别名 L50mt, MRP L50, MRPL50
计算分子量 158 aa, 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC032008
基因名称 MRPL50
Gene ID (NCBI) 54534
RRIDAB_2935441
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N5N7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRPL50, also named as L50mt, is a mitochondrial protein with MW 18 kDa.

实验方案

Product Specific Protocols
WB protocol for MRPL50 antibody 17213-1-APDownload protocol
IHC protocol for MRPL50 antibody 17213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols