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MRPL50 Polyclonal antibody

MRPL50 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17213-1-AP

Print datasheet

Synonyms

L50mt, MRP L50, MRPL50

验证数据展示

  • WB analysis using 17213-1-AP

    WB analysis using 17213-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17213-1-AP (MRPL50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17213-1-AP

    IHC staining of human liver using 17213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17213-1-AP (MRPL50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17213-1-AP is MRPL50 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MRPL50 fusion protein Ag10996 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L50
Synonyms L50mt, MRP L50, MRPL50
Calculated Molecular Weight 158 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC032008
Gene Symbol MRPL50
Gene ID (NCBI) 54534
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5N7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRPL50, also named as L50mt, is a mitochondrial protein with MW 18 kDa.

实验方案

Product Specific Protocols
WB protocol for MRPL50 antibody 17213-1-APDownload protocol
IHC protocol for MRPL50 antibody 17213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols