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MRPS30 Polyclonal antibody

MRPS30 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18441-1-AP

Print datasheet

Synonyms

MRP S30, MRPS30, PAP, PDCD9, S30mt

验证数据展示

  • WB analysis of human myotube protein using 18441-1-AP

    WB analysis of human myotube protein using 18441-1-AP

    WB result of MRPS30 antibody (18441-1-AP, 1:1000) with with 15µg of control human myotube protein by Max Pohjanpelto, University of Helsinki.

  • IF Staining of HepG2 using 18441-1-AP

    IF Staining of HepG2 using 18441-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18441-1-AP (MRPS30 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman myotube protein, HeLa cells, mouse liver mitochondria tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 18441-1-AP is MRPS30 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MRPS30 fusion protein Ag13271 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S30
Synonyms MRP S30, MRPS30, PAP, PDCD9, S30mt
Calculated Molecular Weight 439 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC007735
Gene Symbol MRPS30
Gene ID (NCBI) 10884
RRIDAB_2878544
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS30 antibody 18441-1-APDownload protocol
IF protocol for MRPS30 antibody 18441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

Authors - Liang Wang
View Article
humanWB

Nat Commun

P53-dependent hypusination of eIF5A affects mitochondrial translation and senescence immune surveillance

Authors - Xiangli Jiang
View Article