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  • Featured Product
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MRPS31 Polyclonal antibody

MRPS31 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16288-1-AP

Print datasheet

Synonyms

Small ribosomal subunit protein mS31, S31mt, MRP-S31, MRP S31, IMOGN38

验证数据展示

  • WB analysis using 16288-1-AP

    WB analysis using 16288-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16288-1-AP (MRPS31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16288-1-AP

    WB analysis of HeLa using 16288-1-AP

    WB result of MRPS31 antibody (16288-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS31 transfected HeLa cells.

  • IHC staining of human stomach cancer using 16288-1-AP

    IHC staining of human stomach cancer using 16288-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16288-1-AP (MRPS31 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 16288-1-AP

    IF Staining of HeLa using 16288-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPS31 antibody (16288-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16288-1-AP targets MRPS31 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MRPS31 fusion protein Ag9434 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S31
Synonyms Small ribosomal subunit protein mS31, S31mt, MRP-S31, MRP S31, IMOGN38
Calculated Molecular Weight 395 aa, 45 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC022045
Gene Symbol MRPS31
Gene ID (NCBI) 10240
RRIDAB_2297732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92665
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS31 antibody 16288-1-APDownload protocol
IHC protocol for MRPS31 antibody 16288-1-APDownload protocol
IF protocol for MRPS31 antibody 16288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.

Authors - Juliette Pulman
View Article