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MRPS36 Polyclonal antibody

MRPS36 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16279-1-AP

Print datasheet

Synonyms

S36mt, MRP S36, KGD4, DC47, Alpha-ketoglutarate dehydrogenase subunit 4

验证数据展示

  • IHC staining of human lung cancer using 16279-1-AP

    IHC staining of human lung cancer using 16279-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16279-1-AP (MRPS36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 16279-1-AP

    IF Staining of HeLa using 16279-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16279-1-AP (MRPS36 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16279-1-AP targets MRPS36 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MRPS36 fusion protein Ag9359 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S36
Synonyms S36mt, MRP S36, KGD4, DC47, Alpha-ketoglutarate dehydrogenase subunit 4
Calculated Molecular Weight 103 aa, 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC015966
Gene Symbol MRPS36
Gene ID (NCBI) 92259
RRIDAB_2144247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82909
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for MRPS36 antibody 16279-1-APDownload protocol
IF protocol for MRPS36 antibody 16279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols