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验证数据展示

  • IHC staining of human lung cancer using 16279-1-AP

    IHC staining of human lung cancer using 16279-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16279-1-AP (MRPS36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 16279-1-AP

    IF Staining of HepG2 using 16279-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPS36 antibody (16279-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MRPS36 Polyclonal antibody

MRPS36 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 16279-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

S36mt, MRP S36, KGD4, DC47, Alpha-ketoglutarate dehydrogenase subunit 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16279-1-AP targets MRPS36 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MRPS36 fusion protein Ag9359 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein S36
别名 S36mt, MRP S36, KGD4, DC47, Alpha-ketoglutarate dehydrogenase subunit 4
计算分子量 103 aa, 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC015966
基因名称 MRPS36
Gene ID (NCBI) 92259
RRIDAB_2144247
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP82909
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for MRPS36 antibody 16279-1-APDownload protocol
IF protocol for MRPS36 antibody 16279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols