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MSH3 Recombinant antibody, PBS Only

MSH3 Recombinant Antibody for WB,Indirect ELISA,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

1J23

Cat No : 82845-3-PBS

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Synonyms

Divergent upstream protein, DUC1, DUG, DUP, hMSH3, Mismatch repair protein 1, MRP1, MSH3, mutS homolog 3 (E. coli)

验证数据展示

  • WB analysis using 82845-3-RR (same clone as 82845-3-PBS)

    WB analysis using 82845-3-RR (same clone as 82845-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 82845-3-RR (same clone as 82845-3-PBS)

    IF Staining of U-251 using 82845-3-RR (same clone as 82845-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

产品信息

82845-3-PBS targets MSH3 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human
Immunogen MSH3 fusion protein Ag18060 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mutS homolog 3 (E. coli)
Synonyms Divergent upstream protein, DUC1, DUG, DUP, hMSH3, Mismatch repair protein 1, MRP1, MSH3, mutS homolog 3 (E. coli)
Calculated Molecular Weight 1137 aa, 127 kDa
Observed Molecular Weight 127 kDa
GenBank Accession NumberBC130434
Gene Symbol MSH3
Gene ID (NCBI) 4437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20585
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).