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验证数据展示

  • WB analysis using 82845-3-RR (same clone as 82845-3-PBS)

    WB analysis using 82845-3-RR (same clone as 82845-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82845-3-RR (same clone as 82845-3-PBS)

    WB analysis of HEK-293 using 82845-3-RR (same clone as 82845-3-PBS)

    WB result of MSH3 antibody (82845-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSH3 transfected HEK-293 cells. This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 82845-3-RR (same clone as 82845-3-PBS)

    IF Staining of U-251 using 82845-3-RR (same clone as 82845-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82845-3-PBS

    IF Staining of HeLa using 82845-3-PBS

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH3 antibody (82845-3-PBS, Clone: 1J23 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of U2OS using 82845-3-RR (same clone as 82845-3-PBS)

    IF Staining of U2OS using 82845-3-RR (same clone as 82845-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82845-3-RR

    Affinity and Kinetic Characterization of 82845-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82845-3-RR against Human MSH3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

MSH3 Recombinant antibody, PBS Only

MSH3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82845-3-PBS

产品说明书

CloneNo. 1J23

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, Indirect ELISA

别名

Mismatch repair protein 1, hMSH3, DUG, DUC1, Divergent upstream protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82845-3-PBS targets MSH3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 MSH3 fusion protein Ag18060 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mutS homolog 3 (E. coli)
别名 Mismatch repair protein 1, hMSH3, DUG, DUC1, Divergent upstream protein
计算分子量 1137 aa, 127 kDa
观测分子量 127 kDa
GenBank蛋白编号BC130434
基因名称 MSH3
Gene ID (NCBI) 4437
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20585
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

实验方案

Product Specific Protocols
IF protocol for MSH3 antibody 82845-3-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols