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验证数据展示

  • WB analysis using 82845-3-RR

    WB analysis using 82845-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82845-3-RR

    WB analysis of HEK-293 using 82845-3-RR

    WB result of MSH3 antibody (82845-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSH3 transfected HEK-293 cells.

  • IF Staining of U-251 using 82845-3-RR

    IF Staining of U-251 using 82845-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U2OS using 82845-3-RR

    IF Staining of U2OS using 82845-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 82845-3-RR

    Affinity and Kinetic Characterization of 82845-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82845-3-RR against Human MSH3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

MSH3 Recombinant antibody

MSH3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82845-3-RR

产品说明书

CloneNo. 1J23

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

Mismatch repair protein 1, hMSH3, DUG, DUC1, Divergent upstream protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHSC-T6 cells, HEK-293 cells
Positive IF/ICC detected inU-251 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82845-3-RR targets MSH3 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 MSH3 fusion protein Ag18060 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mutS homolog 3 (E. coli)
别名 Mismatch repair protein 1, hMSH3, DUG, DUC1, Divergent upstream protein
计算分子量 1137 aa, 127 kDa
观测分子量 127 kDa
GenBank蛋白编号BC130434
基因名称 MSH3
Gene ID (NCBI) 4437
RRIDAB_3086552
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20585
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

实验方案

Product Specific Protocols
WB protocol for MSH3 antibody 82845-3-RRDownload protocol
IF protocol for MSH3 antibody 82845-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols