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MSH3 Recombinant antibody

MSH3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1J23

Cat no : 82845-3-RR

Print datasheet

Synonyms

Mismatch repair protein 1, hMSH3, DUG, DUC1, Divergent upstream protein

验证数据展示

  • WB analysis using 82845-3-RR

    WB analysis using 82845-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 82845-3-RR

    IF Staining of U-251 using 82845-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U2OS using 82845-3-RR

    IF Staining of U2OS using 82845-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 82845-3-RR

    Affinity and Kinetic Characterization of 82845-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82845-3-RR against Human MSH3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHSC-T6 cells
Positive IF/ICC detected inU-251 cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82845-3-RR targets MSH3 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, rat
Immunogen MSH3 fusion protein Ag18060 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mutS homolog 3 (E. coli)
Synonyms Mismatch repair protein 1, hMSH3, DUG, DUC1, Divergent upstream protein
Calculated Molecular Weight 1137 aa, 127 kDa
Observed Molecular Weight 127 kDa
GenBank Accession NumberBC130434
Gene Symbol MSH3
Gene ID (NCBI) 4437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20585
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

实验方案

Product Specific Protocols
WB protocol for MSH3 antibody 82845-3-RRDownload protocol
IF protocol for MSH3 antibody 82845-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols