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CoraLite® Plus 488-conjugated MSH3 Recombinant antibody

MSH3 Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1J23

Cat no : CL488-82845-3

Print datasheet

Synonyms

Mismatch repair protein 1, hMSH3, DUG, DUC1, Divergent upstream protein

验证数据展示

  • IF Staining of HeLa using CL488-82845-3

    IF Staining of HeLa using CL488-82845-3

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MSH3 antibody (CL488-82845-3, Clone: 1J23 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 82845-3-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82845-3 targets MSH3 in IF/ICC applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, rat
Immunogen MSH3 fusion protein Ag18060 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mutS homolog 3 (E. coli)
Synonyms Mismatch repair protein 1, hMSH3, DUG, DUC1, Divergent upstream protein
Calculated Molecular Weight 1137 aa, 127 kDa
Observed Molecular Weight 127 kDa
GenBank Accession NumberBC130434
Gene Symbol MSH3
Gene ID (NCBI) 4437
RRIDAB_3673084
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20585
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MSH3 antibody CL488-82845-3Download protocol
Standard Protocols
Click here to view our Standard Protocols