验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 82845-3-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82845-3 targets MSH3 in IF/ICC applications and shows reactivity with human, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | MSH3 fusion protein Ag18060 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | mutS homolog 3 (E. coli) |
| 别名 | Mismatch repair protein 1, hMSH3, DUG, DUC1, Divergent upstream protein |
| 计算分子量 | 1137 aa, 127 kDa |
| 观测分子量 | 127 kDa |
| GenBank蛋白编号 | BC130434 |
| 基因名称 | MSH3 |
| Gene ID (NCBI) | 4437 |
| RRID | AB_3673084 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20585 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MSH3 antibody CL488-82845-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |