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验证数据展示

  • WB analysis using 83744-2-RR

    WB analysis using 83744-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83744-2-RR (MSLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83744-2-RR

    IF Staining of HeLa using 83744-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of THP-1 using 83744-2-RR

    IF Staining of THP-1 using 83744-2-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83744-2-RR

    Affinity and Kinetic Characterization of 83744-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83744-2-RR against Human MSLN were performed. The affinity constant is 1.03 nM.

Mesothelin Recombinant antibody

Mesothelin Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83744-2-RR

产品说明书

CloneNo. 240795G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

MSLN, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor, CAK1 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MKN-45 cells, BxPC-3 cells
Positive IF/ICC detected inHeLa cells, THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83744-2-RR targets Mesothelin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mesothelin
别名 MSLN, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor, CAK1 antigen
计算分子量69KD
观测分子量 69 kDa, 40 kDa
GenBank蛋白编号NM_005823
基因名称 MSLN
Gene ID (NCBI) 10232
RRIDAB_3671343
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13421
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).

实验方案

Product Specific Protocols
WB protocol for Mesothelin antibody 83744-2-RRDownload protocol
IF protocol for Mesothelin antibody 83744-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols