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验证数据展示

  • WB analysis using 83744-2-RR (same clone as 83744-2-PBS)

    WB analysis using 83744-2-RR (same clone as 83744-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83744-2-RR (MSLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00697-2

    Sandwich ELISA standard curve of MP00697-2

    Sandwich ELISA standard curve of MP00697-2, Human MSLN/Mesothelin Recombinant Matched Antibody Pair - PBS only. 83744-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31658. 83744-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP00697-2

    Sample test of MP00697-2

    Urine of four humans was measured. The human MSLN/Mesothelin concentration of detected samples was determined to be 923.5 pg/mL with a range of 138.7 - 1,373.8 pg/mL.

  • Sample test of MP00697-2

    Sample test of MP00697-2

    Saliva of four humans was measured. The human MSLN/Mesothelin concentration of detected samples was determined to be 5,273.4 pg/mL with a range of 209.0 - 16,634.9 pg/mL.

  • Sample test of MP00697-2

    Sample test of MP00697-2

    Serum of four humans was measured. The human MSLN/Mesothelin concentration of detected samples was determined to be 35,341.8 pg/mL with a range of 16,291.9 - 52,246.0 pg/mL.

  • IF Staining of HeLa using 83744-2-RR (same clone as 83744-2-PBS)

    IF Staining of HeLa using 83744-2-RR (same clone as 83744-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 83744-2-RR (same clone as 83744-2-PBS)

    IF Staining of THP-1 using 83744-2-RR (same clone as 83744-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00697-2

    Cytometric bead array standard curve of MP00697-2

    Cytometric bead array standard curve of MP00697-2, MSLN/Mesothelin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83744-5-PBS. Detection antibody: 83744-2-PBS. Standard: Eg31658. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00697-3

    Cytometric bead array standard curve of MP00697-3

    Cytometric bead array standard curve of MP00697-3, MSLN/Mesothelin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83744-3-PBS. Detection antibody: 83744-2-PBS. Standard: Eg31658. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83744-2-RR

    Affinity and Kinetic Characterization of 83744-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83744-2-RR against Human MSLN were performed. The affinity constant is 1.03 nM.

Mesothelin Recombinant antibody, PBS Only (Detector)

Mesothelin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83744-2-PBS

产品说明书

CloneNo. 240795G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

MSLN, 240795G5, CAK1, CAK1 antigen, Megakaryocyte-potentiating factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83744-2-PBS targets Mesothelin as part of a matched antibody pair:

MP00697-2: 83744-5-PBS capture and 83744-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00697-3: 83744-3-PBS capture and 83744-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Mesothelin fusion protein Eg31658 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mesothelin
别名 MSLN, 240795G5, CAK1, CAK1 antigen, Megakaryocyte-potentiating factor
计算分子量69KD
观测分子量 69 kDa, 40 kDa
GenBank蛋白编号NM_005823
基因名称 MSLN
Gene ID (NCBI) 10232
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13421
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).