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验证数据展示

  • WB analysis of HepG2 using 66663-1-Ig (same clone as 66663-1-PBS)

    WB analysis of HepG2 using 66663-1-Ig (same clone as 66663-1-PBS)

    WB result of MST1 antibody (66663-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MST1 transfected HepG2 cells. This data was developed using the same antibody clone with 66663-1-PBS in a different storage buffer formulation.

  • WB analysis using 66663-1-Ig (same clone as 66663-1-PBS)

    WB analysis using 66663-1-Ig (same clone as 66663-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66663-1-Ig (MST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66663-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66663-1-Ig (same clone as 66663-1-PBS)

    IHC staining of human prostate cancer using 66663-1-Ig (same clone as 66663-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66663-1-Ig (MST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66663-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STK4 antibody included
  • IHC staining of human prostate cancer using 66663-1-Ig (same clone as 66663-1-PBS)

    IHC staining of human prostate cancer using 66663-1-Ig (same clone as 66663-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66663-1-Ig (MST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66663-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STK4 antibody included
  • IF Staining of PC-3 using 66663-1-Ig (same clone as 66663-1-PBS)

    IF Staining of PC-3 using 66663-1-Ig (same clone as 66663-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using MST1 antibody (66663-1-Ig, Clone: 2G11C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66663-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MST1 Monoclonal antibody, PBS Only

MST1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66663-1-PBS

产品说明书

CloneNo. 2G11C1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse , Rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DKFZp686A2068, KRS2, MST 1, MST1, serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy STK4 Ready-To-Use IHC Kit. STK4 primary antibody included.

产品信息

66663-1-PBS targets MST1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse , Rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse , Rat
免疫原 MST1 fusion protein Ag17738 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 serine/threonine kinase 4
别名 DKFZp686A2068, KRS2, MST 1, MST1, serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3
计算分子量 487 aa, 56 kDa
观测分子量 52-56 kDa
GenBank蛋白编号BC093768
基因名称 MST1
Gene ID (NCBI) 6789
RRIDAB_2882018
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13043
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.