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- KD/KO Validated
MST1 Monoclonal antibody
MST1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse , Rat and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2G11C1
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, RAW 264.7 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 1 publications below |
产品信息
66663-1-Ig targets MST1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | Human, Mouse , Rat |
Cited Reactivity | human, mouse |
Immunogen | MST1 fusion protein Ag17738 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | serine/threonine kinase 4 |
Synonyms | DKFZp686A2068, KRS2, MST 1, MST1, serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3 |
Calculated Molecular Weight | 487 aa, 56 kDa |
Observed Molecular Weight | 52-56 kDa |
GenBank Accession Number | BC093768 |
Gene Symbol | MST1 |
Gene ID (NCBI) | 6789 |
RRID | AB_2882018 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13043 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MST1 antibody 66663-1-Ig | Download protocol |
IHC protocol for MST1 antibody 66663-1-Ig | Download protocol |
IF protocol for MST1 antibody 66663-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biofactors FERM domain-containing protein 6 exerts a tumor-inhibiting role in thyroid cancer by antagonizing oncogenic YAP1 | ||
J Biol Chem Angiomotins stimulate LATS kinase autophosphorylation and act as scaffolds that promote Hippo signaling. | ||
Biochem Pharmacol A humanized Anti-YKL-40 antibody inhibits tumor development
| ||
Br J Pharmacol Diminished nuclear-localized β-adrenoceptor signalling activates YAP to promote kidney fibrosis in diabetic nephropathy |