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MTA1 Polyclonal antibody, PBS Only

MTA1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30545-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 30545-1-AP (same clone as 30545-1-PBS)

    WB analysis using 30545-1-AP (same clone as 30545-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30545-1-AP (MTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 30545-1-AP (same clone as 30545-1-PBS)

    IHC staining of human prostate cancer using 30545-1-AP (same clone as 30545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTA1 antibody included
  • IHC staining of human prostate cancer using 30545-1-AP (same clone as 30545-1-PBS)

    IHC staining of human prostate cancer using 30545-1-AP (same clone as 30545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTA1 antibody included
  • IF Staining of MCF-7 using 30545-1-AP (same clone as 30545-1-PBS)

    IF Staining of MCF-7 using 30545-1-AP (same clone as 30545-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MTA1 antibody (30545-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 30545-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MTA1 Ready-To-Use IHC Kit. MTA1 primary antibody included.

产品信息

30545-1-PBS targets MTA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen MTA1 fusion protein Ag30994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name metastasis associated 1
Synonyms
Calculated Molecular Weight 81 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberNM_004689
Gene Symbol MTA1
Gene ID (NCBI) 9112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13330
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Metastasis-associated protein 1 (MTA1) is a member of the MTA family which is involved in regulating DNA-based biological processes (PMID: 25332144). MTA1 is an integral part of nucleosome remodeling histone deacetylase complex (NuRD complex) and regulates nucleosome remodeling and deacetylation by stabilizing the NuRD complex and related proteins(PMID: 12419236). MTA1 expression is associated with tumor malignancy in several cancer.