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HRP-conjugated MTAP Monoclonal antibody

MTAP Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB

Conjugate

HRP

CloneNo.

2B1G6

Cat no : HRP-66706

Print datasheet

Synonyms

c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase

验证数据展示

  • WB analysis of NIH/3T3 using HRP-66706

    WB analysis of NIH/3T3 using HRP-66706

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with HRP-66706 (MTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-66706 targets MTAP in WB applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen MTAP fusion protein Ag17954 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name methylthioadenosine phosphorylase
Synonyms c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase
Calculated Molecular Weight 283 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC018625
Gene Symbol MTAP
Gene ID (NCBI) 4507
RRIDAB_2883844
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13126
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

实验方案

Product Specific Protocols
WB protocol for HRP MTAP antibody HRP-66706Download protocol
Standard Protocols
Click here to view our Standard Protocols