验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-66706 targets MTAP in WB applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | MTAP fusion protein Ag17954 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | methylthioadenosine phosphorylase |
| 别名 | c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase |
| 计算分子量 | 283 aa, 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC018625 |
| 基因名称 | MTAP |
| Gene ID (NCBI) | 4507 |
| RRID | AB_2883844 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q13126 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP MTAP antibody HRP-66706 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |