MTCO2 Monoclonal antibody
MTCO2 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
10G14F4
验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, A431 cells, HeLa cells, HepG2 cells, Jurkat cells, LNCaP cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy MTCO2/COX2 Ready-To-Use IHC Kit. MTCO2/COX2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68527-1-Ig targets MTCO2 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA |
| Tested Reactivity | Human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cytochrome c oxidase II |
| Synonyms | COII, COX2, COXII, cytochrome c oxidase II, Cytochrome c oxidase subunit 2, MT CO2, MTCO2 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 23-26 kDa |
| GenBank Accession Number | YP_003024029 |
| Gene Symbol | COX2 |
| Gene ID (NCBI) | 4513 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MTCO2 antibody 68527-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |