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验证数据展示

  • WB analysis using 68524-1-Ig

    WB analysis using 68524-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68524-1-Ig (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68524-1-Ig

    IHC staining of human lung cancer using 68524-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTHFD2 antibody included
  • IHC staining of human lung cancer using 68524-1-Ig

    IHC staining of human lung cancer using 68524-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTHFD2 antibody included

MTHFD2 Monoclonal antibody

MTHFD2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68524-1-Ig

Print datasheet

CloneNo. 1B4B6

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Synonyms

MTHFD2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, LNCaP cells, HCT 116 cells, HeLa cells, HepG2 cells, K-562 cells, U2OS cellls
Positive IHC detected inhuman lung cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MTHFD2 Ready-To-Use IHC Kit. MTHFD2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68524-1-Ig targets MTHFD2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen MTHFD2 fusion protein Ag33605 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Synonyms MTHFD2
Calculated Molecular Weight 350 aa, 38 kDa
Observed Molecular Weight 33-38 kDa
GenBank Accession NumberBC017054
Gene Symbol MTHFD2
Gene ID (NCBI) 10797
RRIDAB_3085232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13995
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).

实验方案

Product Specific Protocols
WB protocol for MTHFD2 antibody 68524-1-IgDownload protocol
IHC protocol for MTHFD2 antibody 68524-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols