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验证数据展示

  • WB analysis using 68524-1-Ig

    WB analysis using 68524-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68524-1-Ig (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68524-1-Ig

    IHC staining of human lung cancer using 68524-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTHFD2 antibody included
  • IHC staining of human lung cancer using 68524-1-Ig

    IHC staining of human lung cancer using 68524-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTHFD2 antibody included

MTHFD2 Monoclonal antibody

MTHFD2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68524-1-Ig

产品说明书

CloneNo. 1B4B6

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

MTHFD2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, LNCaP cells, HCT 116 cells, HeLa cells, HepG2 cells, K-562 cells, U2OS cellls
Positive IHC detected inhuman lung cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MTHFD2 Ready-To-Use IHC Kit. MTHFD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68524-1-Ig targets MTHFD2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 MTHFD2 fusion protein Ag33605 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
别名 MTHFD2
计算分子量 350 aa, 38 kDa
观测分子量 33-38 kDa
GenBank蛋白编号BC017054
基因名称 MTHFD2
Gene ID (NCBI) 10797
RRIDAB_3085232
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13995
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).

实验方案

Product Specific Protocols
WB protocol for MTHFD2 antibody 68524-1-IgDownload protocol
IHC protocol for MTHFD2 antibody 68524-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols