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Host
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 68524-1-Ig (same clone as 68524-1-PBS)

    WB analysis using 68524-1-Ig (same clone as 68524-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68524-1-Ig (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

    IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTHFD2 antibody included
  • IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

    IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTHFD2 antibody included

MTHFD2 Monoclonal antibody, PBS Only

MTHFD2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68524-1-PBS

产品说明书

CloneNo. 1B4B6

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse

应用

WB, IHC, Indirect ELISA

别名

MTHFD2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MTHFD2 Ready-To-Use IHC Kit. MTHFD2 primary antibody included.

产品信息

68524-1-PBS targets MTHFD2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse
免疫原 MTHFD2 fusion protein Ag33605 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
别名 MTHFD2
计算分子量 350 aa, 38 kDa
观测分子量 33-38 kDa
GenBank蛋白编号BC017054
基因名称 MTHFD2
Gene ID (NCBI) 10797
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13995
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).