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MTHFD2 Monoclonal antibody, PBS Only

MTHFD2 Monoclonal Antibody for WB,Indirect ELISA,IHC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

1B4B6

Cat No : 68524-1-PBS

Print datasheet

Synonyms

MTHFD2

验证数据展示

  • WB analysis using 68524-1-Ig (same clone as 68524-1-PBS)

    WB analysis using 68524-1-Ig (same clone as 68524-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68524-1-Ig (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

    IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTHFD2 antibody included
  • IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

    IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTHFD2 antibody included
Planning an IHC experiment? We recommend our IHCeasy MTHFD2 Ready-To-Use IHC Kit. MTHFD2 primary antibody included.

产品信息

68524-1-PBS targets MTHFD2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB,Indirect ELISA,IHC Application Description
Tested Reactivity Human, Mouse
Immunogen MTHFD2 fusion protein Ag33605 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Synonyms MTHFD2
Calculated Molecular Weight 350 aa, 38 kDa
Observed Molecular Weight 33-38 kDa
GenBank Accession NumberBC017054
Gene Symbol MTHFD2
Gene ID (NCBI) 10797
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13995
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).