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验证数据展示

  • WB analysis of Jurkat using 84328-1-RR (same clone as 84328-1-PBS)

    WB analysis of Jurkat using 84328-1-RR (same clone as 84328-1-PBS)

    WB result of MTMR4 antibody (84328-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR4 transfected Jurkat cells. This data was developed using the same antibody clone with 84328-1-PBS in a different storage buffer formulation.

  • WB analysis using 84328-1-RR (same clone as 84328-1-PBS)

    WB analysis using 84328-1-RR (same clone as 84328-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84328-1-RR (MTMR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84328-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84328-1-RR (same clone as 84328-1-PBS)

    IF Staining of HepG2 using 84328-1-RR (same clone as 84328-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTMR4 antibody (84328-1-RR, Clone: 241304E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84328-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84328-1-RR

    Affinity and Kinetic Characterization of 84328-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84328-1-RR against Human MTMR4 were performed. The affinity constant is 2.06 nM.

  • Featured Product
  • KD/KO验证

MTMR4 Recombinant antibody, PBS Only

MTMR4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84328-1-PBS

产品说明书

CloneNo. 241304E11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

KIAA0647, FYVE-DSP2, FYVE DSP2, FYVE domain-containing dual specificity protein phosphatase 2, EC:3.1.3.48

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84328-1-PBS targets MTMR4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 MTMR4 fusion protein Ag4310 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myotubularin related protein 4
别名 KIAA0647, FYVE-DSP2, FYVE DSP2, FYVE domain-containing dual specificity protein phosphatase 2, EC:3.1.3.48
计算分子量 1195 aa, 133 kDa
观测分子量 160-170 kDa
GenBank蛋白编号BC035609
基因名称 MTMR4
Gene ID (NCBI) 9110
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NYA4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048). MTMR4 is an intracellular protein with a calculated molecular mass of 133 kDa, and detected molecular weight in the papers is about 160-170 kDa, which may be due to post-translational modification (PMID: 19125695, PMID: 20736309).