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验证数据展示

  • IF Staining of HepG2 using CL488-27675

    IF Staining of HepG2 using CL488-27675

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 MUC2 antibody (CL488-27675) at dilution of 1:200.

CoraLite® Plus 488-conjugated MUC2 Polyclonal antibody

MUC2 Polyclonal Antibody for IF/ICC
Cat No. CL488-27675

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IF/ICC

Synonyms

Intestinal mucin 2, MLP, MUC 2, MUC2, Mucin 2, SMUC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MUC2 Ready-To-Use IHC Kit. MUC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27675-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27675 targets MUC2 in IF/ICC applications and shows reactivity with Human, Mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse
Immunogen MUC2 fusion protein Ag25800 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mucin 2, oligomeric mucus/gel-forming
Synonyms Intestinal mucin 2, MLP, MUC 2, MUC2, Mucin 2, SMUC
Calculated Molecular Weight 540 kDa
GenBank Accession NumberM94132
Gene Symbol MUC2
Gene ID (NCBI) 4583
RRIDAB_3084111
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MUC2 antibody CL488-27675Download protocol
Standard Protocols
Click here to view our Standard Protocols