验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy MUC2 Ready-To-Use IHC Kit. MUC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27675-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-27675 targets MUC2 in IF/ICC applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse |
| 免疫原 | MUC2 fusion protein Ag25800 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mucin 2, oligomeric mucus/gel-forming |
| 别名 | Intestinal mucin 2, MLP, MUC 2, MUC2, Mucin 2, SMUC |
| 计算分子量 | 540 kDa |
| GenBank蛋白编号 | M94132 |
| 基因名称 | MUC2 |
| Gene ID (NCBI) | 4583 |
| RRID | AB_3084111 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MUC2 antibody CL488-27675 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |