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验证数据展示

  • FC experiment of HeLa using CL488-67351

    FC experiment of HeLa using CL488-67351

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MUS81 (CL488-67351, Clone:2D9B7) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MUS81 Monoclonal antibody

MUS81 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67351

Print datasheet

CloneNo. 2D9B7

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Synonyms

EC:3.1.22.-, 2D9B7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67351-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67351 targets MUS81 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen MUS81 fusion protein Ag29089 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name MUS81 endonuclease homolog (S. cerevisiae)
Synonyms EC:3.1.22.-, 2D9B7
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 52-72 kDa
GenBank Accession NumberBC009999
Gene Symbol MUS81
Gene ID (NCBI) 80198
RRIDAB_2923769
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96NY9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MUS81 antibody CL488-67351Download protocol
Standard Protocols
Click here to view our Standard Protocols