验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, RAW264.7, human brain tissue, mouse lung tissue, rat lung tissue, SH-SY5Y cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue, human testis tissue, human skin tissue, human lung cancer tissue, human kidney tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16478-1-AP targets MVP/LRP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MVP/LRP fusion protein Ag9593 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | major vault protein |
| 别名 | MVP, LRP, VAULT1, Major vault protein, Lung resistance-related protein |
| 计算分子量 | 893 aa, 99 kDa |
| 观测分子量 | 99-104 kDa |
| GenBank蛋白编号 | BC015623 |
| 基因名称 | LRP |
| Gene ID (NCBI) | 9961 |
| RRID | AB_2147597 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14764 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1/p53 axis [PMID: 12894554, 15994960].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| IHC protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| IF protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| IP protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. | ||
Drug Resist Updat CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis
| ||
Adv Sci (Weinh) Tryptophan As a New Member of RNA-Induced Silencing Complexes Prevents Colon Cancer Liver Metastasis | ||
Nat Commun MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression.
| ||
Front Immunol Unraveling the role of Major Vault Protein as a novel immune-related biomarker that promotes the proliferation and migration in pancreatic adenocarcinoma
| ||
Front Cell Dev Biol Major Vault Protein (MVP) Associated With BRAF V600E Mutation Is an Immune Microenvironment-Related Biomarker Promoting the Progression of Papillary Thyroid Cancer via MAPK/ERK and PI3K/AKT Pathways.
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