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  • KD/KO Validated

MVP/LRP Polyclonal antibody

MVP/LRP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16478-1-AP

Print datasheet

Synonyms

MVP, LRP, VAULT1, Major vault protein, Lung resistance-related protein



经过测试的应用

Positive WB detected inA549 cells, RAW264.7, human brain tissue, mouse lung tissue, rat lung tissue, SH-SY5Y cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue, human testis tissue, human skin tissue, human lung cancer tissue, human kidney tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16478-1-AP targets MVP/LRP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MVP/LRP fusion protein Ag9593 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name major vault protein
Synonyms MVP, LRP, VAULT1, Major vault protein, Lung resistance-related protein
Calculated Molecular Weight 893 aa, 99 kDa
Observed Molecular Weight 99-104 kDa
GenBank Accession NumberBC015623
Gene Symbol LRP
Gene ID (NCBI) 9961
RRIDAB_2147597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14764
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1/p53 axis [PMID: 12894554, 15994960].

实验方案

Product Specific Protocols
WB protocol for MVP/LRP antibody 16478-1-APDownload protocol
IHC protocol for MVP/LRP antibody 16478-1-APDownload protocol
IF protocol for MVP/LRP antibody 16478-1-APDownload protocol
IP protocol for MVP/LRP antibody 16478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Authors - Deniz Simsek
humanWB,IHC,IF,IP

Drug Resist Updat

CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis

Authors - Jing Wu
  • KD Validated
humanWB

Nat Commun

MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression.

Authors - Yun Teng
  • KO Validated
mouseWB

Front Cell Dev Biol

Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes.

Authors - Xinlei Li
humanWB

Front Cell Dev Biol

Major Vault Protein (MVP) Associated With BRAF V600E Mutation Is an Immune Microenvironment-Related Biomarker Promoting the Progression of Papillary Thyroid Cancer via MAPK/ERK and PI3K/AKT Pathways.

Authors - Xubin Dong
  • KD Validated
mouseWB

Cell Commun Signal

Differential RNA packaging into small extracellular vesicles by neurons and astrocytes.

Authors - Xuan Luo