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- KD/KO Validated
MVP/LRP Polyclonal antibody
MVP/LRP Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, RAW264.7, human brain tissue, mouse lung tissue, rat lung tissue, SH-SY5Y cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue, human kidney tissue, human testis tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16478-1-AP targets MVP/LRP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA Application Description |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MVP/LRP fusion protein Ag9593 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | major vault protein |
| Synonyms | LRP, major vault protein, MVP, MVP/LRP, VAULT1 |
| Calculated Molecular Weight | 893 aa, 99 kDa |
| Observed Molecular Weight | 99-104 kDa |
| GenBank Accession Number | BC015623 |
| Gene Symbol | MVP |
| Gene ID (NCBI) | 9961 |
| RRID | AB_2147597 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14764 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1/p53 axis [PMID: 12894554, 15994960].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| IHC protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| IF protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| IP protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. | ||
Drug Resist Updat CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis
| ||
Nat Commun MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression.
| ||
Front Cell Dev Biol Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes. | ||
Front Cell Dev Biol Major Vault Protein (MVP) Associated With BRAF V600E Mutation Is an Immune Microenvironment-Related Biomarker Promoting the Progression of Papillary Thyroid Cancer via MAPK/ERK and PI3K/AKT Pathways.
| ||
Cell Commun Signal Differential RNA packaging into small extracellular vesicles by neurons and astrocytes. |
