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验证数据展示

  • IF Staining of HepG2 using CL488-16478

    IF Staining of HepG2 using CL488-16478

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 MVP/LRP antibody (CL488-16478) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-16478

    FC experiment of HepG2 using CL488-16478

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 MVP/LRP Polyclonal Antibody (CL488-16478)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated MVP/LRP Polyclonal antibody

MVP/LRP Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-16478

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

LRP, major vault protein, MVP, MVP/LRP, VAULT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 16478-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16478 targets MVP/LRP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 MVP/LRP fusion protein Ag9593 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 major vault protein
别名 LRP, major vault protein, MVP, MVP/LRP, VAULT1
计算分子量 893 aa, 99 kDa
观测分子量 99-104 kDa
GenBank蛋白编号BC015623
基因名称 LRP
Gene ID (NCBI) 9961
RRIDAB_3672655
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14764
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1/p53 axis [PMID: 12894554, 15994960].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MVP/LRP antibody CL488-16478Download protocol
FC protocol for CL Plus 488 MVP/LRP antibody CL488-16478Download protocol
Standard Protocols
Click here to view our Standard Protocols