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验证数据展示

  • WB analysis using 82677-1-RR

    WB analysis using 82677-1-RR

    Untreated and IFN alpha treated A549 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with 82677-1-RR (MX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 82677-1-RR

    IHC staining of human lung cancer using 82677-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 82677-1-RR

    IHC staining of human lung cancer using 82677-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 82677-1-RR

    IHC staining of human tonsillitis using 82677-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 82677-1-RR

    IHC staining of human tonsillitis using 82677-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 82677-1-RR

    IF Staining of A549 using 82677-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MX1 antibody (82677-1-RR, Clone: 2L19 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82677-1-RR

    Affinity and Kinetic Characterization of 82677-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82677-1-RR against Human MX1 were performed. The affinity constant is 0.763 nM.

MX1 Recombinant antibody

MX1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82677-1-RR

产品说明书

CloneNo. 2L19

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Interferon-regulated resistance GTP-binding protein MxA, Interferon-induced protein p78, Interferon-induced GTP-binding protein Mx1, N-terminally processed, Interferon-induced GTP-binding protein Mx1, IFI-78K

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inIFN alpha treated A549 cells, IFN alpha treated HepG2 cells
Positive IHC detected inhuman lung cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82677-1-RR targets MX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MX1 fusion protein Ag4692 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Myxovirus resistance protein 1
别名 Interferon-regulated resistance GTP-binding protein MxA, Interferon-induced protein p78, Interferon-induced GTP-binding protein Mx1, N-terminally processed, Interferon-induced GTP-binding protein Mx1, IFI-78K
计算分子量 662 aa, 76 kDa
观测分子量 75 kDa
GenBank蛋白编号BC032602
基因名称 MX1
Gene ID (NCBI) 4599
RRIDAB_3086503
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20591
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN.

实验方案

Product Specific Protocols
WB protocol for MX1 antibody 82677-1-RRDownload protocol
IHC protocol for MX1 antibody 82677-1-RRDownload protocol
IF protocol for MX1 antibody 82677-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols