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CoraLite® Plus 488-conjugated MX1 Recombinant antibody

MX1 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2L19

Cat No : CL488-82677

Print datasheet

Synonyms

IFI 78K, IFI78, Interferon induced protein p78, MX, MX1, MxA, Myxovirus resistance protein 1

验证数据展示

  • IF Staining of A431 using CL488-82677

    IF Staining of A431 using CL488-82677

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 MX1 antibody (CL488-82677, Clone: 2L19 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 82677-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82677 targets MX1 in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen MX1 fusion protein Ag4692 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Myxovirus resistance protein 1
Synonyms IFI 78K, IFI78, Interferon induced protein p78, MX, MX1, MxA, Myxovirus resistance protein 1
Calculated Molecular Weight 662 aa, 76 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC032602
Gene Symbol MX1
Gene ID (NCBI) 4599
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20591
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MX1 antibody CL488-82677Download protocol
Standard Protocols
Click here to view our Standard Protocols