MYCN Recombinant antibody
MYCN Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
242406D8
验证数据展示
经过测试的应用
| Positive WB detected in | IMR-32 cells, human placenta tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84886-3-RR targets MYCN in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, FC (Intra), ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | MYCN fusion protein Ag27224 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) |
| Synonyms | N-myc proto-oncogene protein, N myc proto oncogene protein, N myc, MODED, bHLHe37 |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | ~65 kDa |
| GenBank Accession Number | BC002712 |
| Gene Symbol | MYCN |
| Gene ID (NCBI) | 4613 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04198 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYCN, also named as BHLHE37 and NMYC, is a transcription factor. It is primarily expressed in normal developing embryos and is thought to be critical in brain and other neural development. It is often amplified in human neuroblastomas. IR34A can suppress MYCN expression, it suggest MYCN has a role in cell growth. The expression of the MCYN is upregulated in a variety of human tumors, most frequently neuroblastoma, where the level of expression appears to increase as the tumor progresses. The calculated molecuar weight of MYCN is 50 kDa, but Phosphorylated MYCN is about 65-70 kDa. MYCN exsits two isoforms and MV of isoform is respectively 65 kDa and 45 kDa. (PMID: 19615087 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYCN antibody 84886-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
