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CoraLite® Plus 488-conjugated MYD88 Monoclonal antibody

MYD88 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E10D3

Cat no : CL488-67969

Print datasheet

Synonyms

2E10D3

验证数据展示

  • FC experiment of K-562 using CL488-67969

    FC experiment of K-562 using CL488-67969

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MYD88 (CL488-67969, Clone:2E10D3) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy MYD88 Ready-To-Use IHC Kit. MYD88 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67969-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67969 targets MYD88 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen MYD88 fusion protein Ag27659 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name myeloid differentiation primary response gene (88)
Synonyms 2E10D3
Calculated Molecular Weight 33 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC013589
Gene Symbol MYD88
Gene ID (NCBI) 4615
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99836
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYD88 is a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. MYD88 is the key adaptor protein for the interleukin-1 receptor (IL-1R) and most Toll-like receptors (TLRs), which are essential for innate immunity and pathogen-associated molecular pattern recognition. Mutations in the MyD88 gene lead to the development of cancer in humans and mice suggesting that MyD88 also plays a cell autonomous role in tissue homeostasis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MYD88 antibody CL488-67969Download protocol
Standard Protocols
Click here to view our Standard Protocols