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MYH1 Monoclonal antibody, PBS Only

MYH1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10H9

Cat no : 67299-1-PBS

Print datasheet

Synonyms

MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d

验证数据展示

  • IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH1 antibody included
  • IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH1 antibody included
  • WB analysis of human skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    WB analysis of human skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    1 μg human skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    IF Staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH1 antibody (67299-1-Ig, Clone: 1G10H9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.

产品信息

67299-1-PBS targets MYH1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, mouse, pig
Immunogen MYH1 fusion protein Ag17129 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name myosin, heavy chain 1, skeletal muscle, adult
Synonyms MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d
Calculated Molecular Weight 1939 aa, 223 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC114545
Gene Symbol MYH1
Gene ID (NCBI) 4619
RRIDAB_2882563
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12882
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.