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验证数据展示

  • IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH1 antibody included
  • IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH1 antibody included
  • WB analysis of human skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    WB analysis of human skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    1 μg human skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

  • IHC staining of rat skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    IHC staining of rat skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:80000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH1 antibody included
  • IF Staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    IF Staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH1 antibody (67299-1-Ig, Clone: 1G10H9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

MYH1 Monoclonal antibody, PBS Only

MYH1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67299-1-PBS

产品说明书

CloneNo. 1G10H9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, pig

应用

WB, IHC, IF-P, Indirect ELISA

别名

Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x, 1G10H9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.

产品信息

67299-1-PBS targets MYH1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, pig
免疫原 MYH1 fusion protein Ag17129 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, heavy chain 1, skeletal muscle, adult
别名 Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x, 1G10H9
计算分子量 1939 aa, 223 kDa
观测分子量 220 kDa
GenBank蛋白编号BC114545
基因名称 MYH1
Gene ID (NCBI) 4619
RRIDAB_2882563
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12882
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.