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MYH1 Monoclonal antibody, PBS Only

MYH1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10H9

Cat No : 67299-1-PBS

Print datasheet

Synonyms

MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d

验证数据展示

  • IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH1 antibody included
  • IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH1 antibody included
  • WB analysis of human skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    WB analysis of human skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    1 μg human skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    IF Staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH1 antibody (67299-1-Ig, Clone: 1G10H9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.

产品信息

67299-1-PBS targets MYH1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, pig
Immunogen MYH1 fusion protein Ag17129 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name myosin, heavy chain 1, skeletal muscle, adult
Synonyms MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d
Calculated Molecular Weight 1939 aa, 223 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC114545
Gene Symbol MYH1
Gene ID (NCBI) 4619
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12882
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.