MYH1 Monoclonal antibody

MYH1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67299-1-Ig

产品说明书

CloneNo. 1G10H9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, pig and More (1)

应用

WB, IHC, IF-P, ELISA and More (1)

Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x, 1G10H9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, pig skeletal muscle tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2000-1:20000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67299-1-Ig targets MYH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, pig
文献引用反应性human, mouse, pig, chicken
免疫原 MYH1 fusion protein Ag17129 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, heavy chain 1, skeletal muscle, adult
别名 Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x, 1G10H9
计算分子量 1939 aa, 223 kDa
观测分子量 220 kDa
GenBank蛋白编号BC114545
基因名称 MYH1
Gene ID (NCBI) 4619
RRIDAB_2882563
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12882
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

实验方案

Product Specific Protocols
WB protocol for MYH1 antibody 67299-1-IgDownload protocol
IHC protocol for MYH1 antibody 67299-1-IgDownload protocol
IF protocol for MYH1 antibody 67299-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

BMC Genomics

Identification of different myofiber types in pigs muscles and construction of regulatory networks

Authors - Chenchen Li
pigWB

Food Funct

Potential nutritional healthy-aging strategy: enhanced protein metabolism by balancing branched-chain amino acids in a finishing pig model.

Authors - Lingyu Zhang
humanIHC

Pathol Res Pract

Layer-specific proteomic profiling of human normal heart

Authors - Yu Kakimoto
pigWB

J Biol Chem

Circular RNA circMYLK4 shifts energy metabolism from glycolysis to OXPHOS by binding to the calcium channel auxiliary subunit CACNA2D2

Authors - Haigang Cao
mouseIF,WB

Int J Mol Sci

Endurance Exercise-Induced Fgf21 Promotes Skeletal Muscle Fiber Conversion through TGF-β1 and p38 MAPK Signaling Pathway

Authors - Xiaomao Luo
mouseWB,IF

Aging (Albany NY)

Myogenic exosome miR-140-5p modulates skeletal muscle regeneration and injury repair by regulating muscle satellite cells

Authors - Xiaorui Cao
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