验证数据展示
经过测试的应用
Positive IF-P detected in | mouse skeletal muscle tissue |
Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67299-1-Ig
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67299 targets MYH1 in IF-P applications and shows reactivity with Human, mouse, pig samples.
经测试应用 | IF-P Application Description |
经测试反应性 | Human, mouse, pig |
免疫原 | MYH1 fusion protein Ag17129 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | myosin, heavy chain 1, skeletal muscle, adult |
别名 | MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d |
计算分子量 | 1939 aa, 223 kDa |
观测分子量 | 220 kDa |
GenBank蛋白编号 | BC114545 |
基因名称 | MYH1 |
Gene ID (NCBI) | 4619 |
RRID | AB_3084334 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P12882 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 MYH1 antibody CL488-67299 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |