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验证数据展示

  • IF Staining of mouse skeletal muscle using CL488-67299

    IF Staining of mouse skeletal muscle using CL488-67299

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite® Plus 488 MYH1 antibody (CL488-67299, Clone: 1G10H9 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated MYH1 Monoclonal antibody

MYH1 Monoclonal Antibody for IF-P
Cat No. CL488-67299

Print datasheet

CloneNo. 1G10H9

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

IF-P

Synonyms

MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67299-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67299 targets MYH1 in IF-P applications and shows reactivity with Human, mouse, pig samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, mouse, pig
Immunogen MYH1 fusion protein Ag17129 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name myosin, heavy chain 1, skeletal muscle, adult
Synonyms MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d
Calculated Molecular Weight 1939 aa, 223 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC114545
Gene Symbol MYH1
Gene ID (NCBI) 4619
RRIDAB_3084334
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12882
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MYH1 antibody CL488-67299Download protocol
Standard Protocols
Click here to view our Standard Protocols