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MYH1-motor domain Polyclonal antibody, PBS Only

MYH1-motor domain Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 22282-1-PBS

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Synonyms

验证数据展示

  • WB analysis of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    WB analysis of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22282-1-AP (MYH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

  • IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH1 antibody included
  • IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH1 antibody included
  • FC experiment of C2C12 using 22282-1-AP (same clone as 22282-1-PBS)

    FC experiment of C2C12 using 22282-1-AP (same clone as 22282-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYH1-motor domain (22282-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.

产品信息

22282-1-PBS targets MYH1-motor domain in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, heavy chain 1, skeletal muscle, adult
Synonyms
Calculated Molecular Weight 223 kDa
Observed Molecular Weight 223 kDa
GenBank Accession NumberNM_005963
Gene Symbol MYH1
Gene ID (NCBI) 4619
RRIDAB_2879059
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12882
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.