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验证数据展示

  • WB analysis of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    WB analysis of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22282-1-AP (MYH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

  • IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH1 antibody included
  • IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH1 antibody included
  • FC experiment of C2C12 using 22282-1-AP (same clone as 22282-1-PBS)

    FC experiment of C2C12 using 22282-1-AP (same clone as 22282-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYH1-motor domain (22282-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

MYH1-motor domain Polyclonal antibody, PBS Only

MYH1-motor domain Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 22282-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.

产品信息

22282-1-PBS targets MYH1-motor domain in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin, heavy chain 1, skeletal muscle, adult
别名
计算分子量 223 kDa
观测分子量 223 kDa
GenBank蛋白编号NM_005963
基因名称 MYH1
Gene ID (NCBI) 4619
RRIDAB_2879059
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12882
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.