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  • Featured Product
  • KD/KO Validated

MYH10 Monoclonal antibody, PBS Only

MYH10 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H8G7

Cat no : 67243-1-PBS

Print datasheet

Synonyms

MYH10, Myosin 10, Myosin heavy chain 10

验证数据展示

  • WB analysis of mouse brain using 67243-1-Ig (same clone as 67243-1-PBS)

    WB analysis of mouse brain using 67243-1-Ig (same clone as 67243-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

    IHC staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67243-1-Ig (MYH10 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH10 antibody included
  • IF Staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

    IF Staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using MYH10 antibody (67243-1-Ig, Clone: 2H8G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

    IHC staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67243-1-Ig (MYH10 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH10 antibody included
  • WB analysis of HEK-293 using 67243-1-Ig (same clone as 67243-1-PBS)

    WB analysis of HEK-293 using 67243-1-Ig (same clone as 67243-1-PBS)

    WB result of MYH10 antibody (67243-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYH10 transfected HEK-293 cells. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MYH10 Ready-To-Use IHC Kit. MYH10 primary antibody included.

产品信息

67243-1-PBS targets MYH10 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen MYH10 fusion protein Ag17069 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name myosin, heavy chain 10, non-muscle
Synonyms MYH10, Myosin 10, Myosin heavy chain 10
Calculated Molecular Weight 1985 aa, 230 kDa
Observed Molecular Weight 200-230 kDa
GenBank Accession NumberBC150634
Gene Symbol MYH10
Gene ID (NCBI) 4628
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35580
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.