验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue, rat cerebellum tissue, mouse cerebellum tissue |
Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse cerebellum tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67243-1-Ig targets MYH10 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, IHC, IF-P, ELISA Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | MYH10 fusion protein Ag17069 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | myosin, heavy chain 10, non-muscle |
别名 | MYH10, Myosin 10, Myosin heavy chain 10 |
计算分子量 | 1985 aa, 230 kDa |
观测分子量 | 200-230 kDa |
GenBank蛋白编号 | BC150634 |
基因名称 | MYH10 |
Gene ID (NCBI) | 4628 |
RRID | AB_2882522 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P35580 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.
实验方案
Product Specific Protocols | |
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WB protocol for MYH10 antibody 67243-1-Ig | Download protocol |
IHC protocol for MYH10 antibody 67243-1-Ig | Download protocol |
IF protocol for MYH10 antibody 67243-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |