验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue, rat cerebellum tissue, mouse cerebellum tissue |
Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse cerebellum tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67243-1-Ig targets MYH10 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | MYH10 fusion protein Ag17069 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | myosin, heavy chain 10, non-muscle |
Synonyms | MYH10, Myosin 10, Myosin heavy chain 10 |
Calculated Molecular Weight | 1985 aa, 230 kDa |
Observed Molecular Weight | 200-230 kDa |
GenBank Accession Number | BC150634 |
Gene Symbol | MYH10 |
Gene ID (NCBI) | 4628 |
RRID | AB_2882522 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P35580 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.
实验方案
Product Specific Protocols | |
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WB protocol for MYH10 antibody 67243-1-Ig | Download protocol |
IHC protocol for MYH10 antibody 67243-1-Ig | Download protocol |
IF protocol for MYH10 antibody 67243-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |