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MYH10 Monoclonal antibody

MYH10 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2H8G7

Cat no : 67243-1-Ig

Print datasheet

Synonyms

MYH10, Myosin 10, Myosin heavy chain 10

验证数据展示

  • WB analysis of mouse brain using 67243-1-Ig

    WB analysis of mouse brain using 67243-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 67243-1-Ig

    IHC staining of mouse cerebellum using 67243-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67243-1-Ig (MYH10 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH10 antibody included
  • IF Staining of mouse cerebellum using 67243-1-Ig

    IF Staining of mouse cerebellum using 67243-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using MYH10 antibody (67243-1-Ig, Clone: 2H8G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse cerebellum using 67243-1-Ig

    IHC staining of mouse cerebellum using 67243-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67243-1-Ig (MYH10 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH10 antibody included
  • WB analysis of HEK-293 using 67243-1-Ig

    WB analysis of HEK-293 using 67243-1-Ig

    WB result of MYH10 antibody (67243-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYH10 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy MYH10 Ready-To-Use IHC Kit. MYH10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67243-1-Ig targets MYH10 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen MYH10 fusion protein Ag17069 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name myosin, heavy chain 10, non-muscle
Synonyms MYH10, Myosin 10, Myosin heavy chain 10
Calculated Molecular Weight 1985 aa, 230 kDa
Observed Molecular Weight 200-230 kDa
GenBank Accession NumberBC150634
Gene Symbol MYH10
Gene ID (NCBI) 4628
RRIDAB_2882522
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35580
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.

实验方案

Product Specific Protocols
WB protocol for MYH10 antibody 67243-1-IgDownload protocol
IHC protocol for MYH10 antibody 67243-1-IgDownload protocol
IF protocol for MYH10 antibody 67243-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols