验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse cerebellum tissue |
Planning an IHC experiment? We recommend our IHCeasy MYH10 Ready-To-Use IHC Kit. MYH10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67243-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-67243 targets MYH10 in IF-P applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | MYH10 fusion protein Ag17069 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | myosin, heavy chain 10, non-muscle |
| 别名 | MYH10, Myosin 10, Myosin heavy chain 10 |
| 计算分子量 | 1985 aa, 230 kDa |
| 观测分子量 | 200-230 kDa |
| GenBank蛋白编号 | BC150634 |
| 基因名称 | MYH10 |
| Gene ID (NCBI) | 4628 |
| RRID | AB_2920102 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35580 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 MYH10 antibody CL594-67243 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |