验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse cerebellum tissue |
Planning an IHC experiment? We recommend our IHCeasy MYH10 Ready-To-Use IHC Kit. MYH10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67243-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-67243 targets MYH10 in IF-P applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF-P Application Description |
| Cited Applications | IF |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | MYH10 fusion protein Ag17069 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | myosin, heavy chain 10, non-muscle |
| Synonyms | MYH10, Myosin 10, Myosin heavy chain 10 |
| Calculated Molecular Weight | 1985 aa, 230 kDa |
| Observed Molecular Weight | 200-230 kDa |
| GenBank Accession Number | BC150634 |
| Gene Symbol | MYH10 |
| Gene ID (NCBI) | 4628 |
| RRID | AB_2920102 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35580 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 MYH10 antibody CL594-67243 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |