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MYH9 Recombinant antibody, PBS Only

MYH9 Recombinant Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3E1

Cat No : 81204-1-PBS

Print datasheet

Synonyms

DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9

验证数据展示

  • WB analysis using 81204-1-RR (same clone as 81204-1-PBS)

    WB analysis using 81204-1-RR (same clone as 81204-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81204-1-RR (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81204-1-RR (same clone as 81204-1-PBS)

    IHC staining of human colon cancer using 81204-1-RR (same clone as 81204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81204-1-RR (MYH9 antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH9 antibody included
  • IHC staining of rat kidney using 81204-1-RR (same clone as 81204-1-PBS)

    IHC staining of rat kidney using 81204-1-RR (same clone as 81204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 81204-1-RR (MYH9 antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH9 antibody included
  • IF Staining of HepG2 using 81204-1-RR (same clone as 81204-1-PBS)

    IF Staining of HepG2 using 81204-1-RR (same clone as 81204-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH9 antibody (81204-1-RR, Clone: 3E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MYH9 Ready-To-Use IHC Kit. MYH9 primary antibody included.

产品信息

81204-1-PBS targets MYH9 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen MYH9 fusion protein Ag1612 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myosin, heavy chain 9, non-muscle
Synonyms DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9
Calculated Molecular Weight 227 kDa
Observed Molecular Weight224-227 kDa
GenBank Accession NumberBC011915
Gene Symbol MYH9
Gene ID (NCBI) 4627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35579
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).